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pET19b-MED17
(Plasmid #15412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET19b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MED17
  • Alt name
    TRAP80
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1956
  • Mutation
    N/A
  • GenBank ID
    BE883062
  • Entrez Gene
    MED17 (a.k.a. CRSP6, CRSP77, DRIP80, TRAP80)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET19b-MED17 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15412 ; http://n2t.net/addgene:15412 ; RRID:Addgene_15412)

  • For your References section:

    A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2003 Dec 12. 278(50):49671-4. 10.1074/jbc.C300444200 PubMed 14576168
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