pNM3649
(Plasmid #154110)

• Purpose: miniMos dual component RMCE landing site vector
• Depositing Lab: Michael Nonet
• Publication: Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.

Backbone

• Vector backbone: pCFJ1272
• Backbone manufacturer: Addgene #44486
• Backbone size w/o insert (bp): 3173
• Total vector size (bp): 11235
• Vector type: miniMos
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
• Species: C. elegans (nematode), S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 8086
• Promoter: not applicable

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: not applicable

Resource Information

• Supplemental Documents:
  • pNM3649.gb
• A portion of this plasmid was derived from a plasmid made by: The insert was assembled using a multi-fragment Golden Gate reaction into an intermediate clone then moved into pCFJ1272 using standard restriction enzyme cloning. The insert was assembled from loxP::SEC::lopP from Addgene# 91829, rpl-28p from genomic sequences, FRT as a synthetic DNA, GFP::His-58::unc-54 from Addgene #86718, and FRT3 as a synthetic DNA. See Nonet (2020) Genetics for precise details.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry