pSCIPpay-BFP reporter
(Plasmid #154096)

• Purpose: Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter
• Depositing Lab: Scott McComb
• Publication: Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10.1089/crispr.2020.0090.

Backbone

• Vector backbone: pQCi
• Backbone manufacturer: Derived from pX458 (Zhang lab)
• Backbone size w/o insert (bp): 9500
• Total vector size (bp): 10164
• Modifications to backbone: Modular BAIT sites at 5' and 3' end of P2A-tagBFP (BsaI sites) Intron integrated into spCas9 sequence
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: P2A-tagBFP
• Alt name: Blue fluorescent protein
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pSCIPpay-BFP Reporter.gb
  • Protocol for cloning sgRNA and BAIT sequences to make a SCIPpay-BFP-reporter plasmid
  • Excel sheet for helping design sgRNA and BAIT oligos for SCIPpay-BFP reporter
• A portion of this plasmid was derived from a plasmid made by: https://www.addgene.org/48138/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.03.25.008276v4 for bioRxiv preprint.