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pBS1479-HTP-KanMX6
(Plasmid #154085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS1479
  • Total vector size (bp) 4825
  • Vector type
    PCR-based tagging in yeast
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    his6-TEV-protein A-KanR
  • Species
    Synthetic
  • Insert Size (bp)
    1953
  • Tag / Fusion Protein
    • his6-TEV-protein A (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer 2686-pFA6arev
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    KanMX6 cassette from pFA6a-KanMX6 (Bähler et al. (1998) Yeast, 14(10):943-51) inserted into pBS1479-HTP-TRP1 (Granneman et al. (2009) PNAS, 106(24): 9613-9618) to replace TRP1 marker with KanMX6.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS1479-HTP-KanMX6 was a gift from Lidia Vasiljeva (Addgene plasmid # 154085 ; http://n2t.net/addgene:154085 ; RRID:Addgene_154085)

  • For your References section:

    Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1. Kilchert C, Wittmann S, Passoni M, Shah S, Granneman S, Vasiljeva L. Cell Rep. 2015 Dec 22;13(11):2504-2515. doi: 10.1016/j.celrep.2015.11.026. Epub 2015 Dec 6. 10.1016/j.celrep.2015.11.026 PubMed 26670050
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