mOrange-P2A-hKvb1
(Plasmid #154084)

• Purpose: Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter
• Depositing Lab: Michael Hoppa
• Publication: Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29937-29947. doi: 10.1073/pnas.2000790117. Epub 2020 Nov 9.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4579
• Total vector size (bp): 6559
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mOrange-P2A-human Kvb1
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1980
• GenBank ID: NM172159.2
• Entrez Gene: KCNAB1 (a.k.a. AKR6A3, KCNA1B, KV-BETA-1, Kvb1.3, hKvBeta3, hKvb3)
• Promoter: human synapsin 1

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CAAACTCCCCTTCCCGGCCA
• 3′ sequencing primer: ggcattaaagcagcgtatcc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry