pMG059 MBP-CREB-His
(Plasmid #154076)

• Purpose: Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli
• Depositing Lab: Jesse Zalatan
• Publication: Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.

Backbone

• Vector backbone: pMBP-MG
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 6716
• Total vector size (bp): 7739
• Modifications to backbone: Modified to include an N-terminal TEV-cleavable MBP tag and a C-terminal His6 tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CREB1
• Alt name: Cyclic AMP-responsive element-binding protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 1023
• GenBank ID: M34356
• Entrez Gene: CREB1 (a.k.a. CREB, CREB-1)
• Promoter: Tac
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: MalE Forward GGTCGTCAGACTGTCGATGAAGCC
• 3′ sequencing primer: M13F Reverse TGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pMG059_pMBP_CREB_FL.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene #82203

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry