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Addgene

SARS-COV2-NSP1-HA-BASU
(Plasmid #154071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF1-alpha-promoter
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp1
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-COV2-NSP1-HA-BASU was a gift from Paul Khavari (Addgene plasmid # 154071 ; http://n2t.net/addgene:154071 ; RRID:Addgene_154071)

  • For your References section:

    The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation. Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, Blish C, Wang Y, Khavari PA. PLoS Pathog. 2021 Oct 1;17(10):e1009412. doi: 10.1371/journal.ppat.1009412. 10.1371/journal.ppat.1009412 PubMed 34597346
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