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Addgene

FLAG-MED9
(Plasmid #15407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15407 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 N-FLAG/Hyg
  • Backbone manufacturer
    Invitrogen, modified at Conaway lab
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MED9
  • Alt name
    FLJ10193
  • Alt name
    Med25
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    429
  • Mutation
    N/A
  • GenBank ID
    NM_138675
  • Entrez Gene
    Med9 (a.k.a. Med25, BC019367, FLJ10193, MGC30366)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-MED9 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15407 ; http://n2t.net/addgene:15407 ; RRID:Addgene_15407)

  • For your References section:

    A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2004 Feb 13. 279(7):5846-51. 10.1074/jbc.M312523200 PubMed 14638676
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