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Addgene

EC71102
(Plasmid #154065)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMS (standard backbone from GENEART, Thermofisher)
  • Backbone manufacturer
    ENSA/ Invitrogen synthesis
  • Backbone size w/o insert (bp) 2609
  • Total vector size (bp) 3676
  • Vector type
    Synthetic Biology ; Standard cloning vector used by GENEART for synthesis products

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE
  • Alt name
    Cre Recombinase
  • Species
    Enterobacteria phage P1
  • Insert Size (bp)
    1037
  • Mutation
    All BsaI, Esp3I and BPiI restriction sites were removed using synonymous changes, BsaI restriction sites are added at each end
  • GenBank ID
    NM_2777477

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthesised using GENEART

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC71102 was a gift from Cristobal Uauy (Addgene plasmid # 154065 ; http://n2t.net/addgene:154065 ; RRID:Addgene_154065)

  • For your References section:

    A heat-shock inducible system for flexible gene expression in cereals. Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, Richardson A. Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. eCollection 2020. 10.1186/s13007-020-00677-3 PubMed 33072173
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