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Addgene

enz.099-PKA
(Plasmid #154048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAJM.029
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 5201
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Protein kinase cAMP-activated catalytic subunit alpha (PKA)
  • Alt name
    PKACA
  • Alt name
    PRKACA
  • Alt name
    PPNAD4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2244
  • GenBank ID
    5566
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Promoter T7
  • Tag / Fusion Protein
    • His, MBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAATTCGCTGCAGCTTCTAGAGTAATACG
  • 3′ sequencing primer GCTACTAGTATATAAACGCAGAAAGGCCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    enz.099-PKA was a gift from Christopher Voigt (Addgene plasmid # 154048 ; http://n2t.net/addgene:154048 ; RRID:Addgene_154048)

  • For your References section:

    Silica Nanostructures Produced Using Diatom Peptides with Designed Post-Translational Modifications. Wallace AK, Chanut N, Voigt CA. Advanced Functional Materials 2020 10.1002/adfm.202000849
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