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Addgene

enz.079-GCN5
(Plasmid #154046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAJM.029
  • Backbone size w/o insert (bp) 2922
  • Total vector size (bp) 5454
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Histone acetyltransferase GCN5
  • Alt name
    GCN5
  • Alt name
    His-MBP-TEV-GCN5
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2505
  • Entrez Gene
    GCN5 (a.k.a. YGR252W, AAS104, ADA4, KAT2, SWI9)
  • Promoter T7
  • Tag / Fusion Protein
    • His, MBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAATTCGCTGCAGCTTCTAGAGTAATACG
  • 3′ sequencing primer GCTACTAGTATATAAACGCAGAAAGGCCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    enz.079-GCN5 was a gift from Christopher Voigt (Addgene plasmid # 154046 ; http://n2t.net/addgene:154046 ; RRID:Addgene_154046)

  • For your References section:

    Silica Nanostructures Produced Using Diatom Peptides with Designed Post-Translational Modifications. Wallace AK, Chanut N, Voigt CA. Advanced Functional Materials 2020 10.1002/adfm.202000849
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