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Addgene

pAKW.031-GM-R5
(Plasmid #154044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 2385
  • Total vector size (bp) 3653
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Silaffin R5 peptide
  • Alt name
    MBP-His-TEV-R5
  • Alt name
    R5
  • Species
    Cylindrotheca fusiformis
  • Insert Size (bp)
    1236
  • GenBank ID
    AF191634
  • Promoter T7
  • Tag / Fusion Protein
    • Maltose binding protein and his tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATCGGTGATGTCGGCGATATAG
  • 3′ sequencing primer CACACCCGCCGCGCTTAATGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKW.031-GM-R5 was a gift from Christopher Voigt (Addgene plasmid # 154044 ; http://n2t.net/addgene:154044 ; RRID:Addgene_154044)

  • For your References section:

    Silica Nanostructures Produced Using Diatom Peptides with Designed Post-Translational Modifications. Wallace AK, Chanut N, Voigt CA. Advanced Functional Materials 2020 10.1002/adfm.202000849
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