pL0_EF_tPbt
(Plasmid
#154043)
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PurposeDiatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepL0RlacZ
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTerminator Pbt
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SpeciesP. tricornutum
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL0_EF_tPbt was a gift from Raffaela Abbriano (Addgene plasmid # 154043 ; http://n2t.net/addgene:154043 ; RRID:Addgene_154043) -
For your References section:
Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum. Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.. Algal Research , Volume 53, March 2021 10.1016/j.algal.2020.102159