pBlue-hFX-HA(AscI)
(Plasmid
#15404)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman frataxin cDNA
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Alt namehFX
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Alt nameFRX
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Alt namefrataxin
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SpeciesH. sapiens (human)
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Insert Size (bp)699
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Mutation700 bp insert can be released by digestion with restriction enzyme AscI
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Entrez GeneFXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
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Tags
/ Fusion Proteins
- HA (C terminal on insert)
- AU1 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlue-hFX-HA(AscI) was a gift from Michael Ristow (Addgene plasmid # 15404 ; http://n2t.net/addgene:15404 ; RRID:Addgene_15404)