pBlue-hFX(I154F)(AA88)-HA
(Plasmid #15403)

• Depositing Lab: Michael Ristow
• Publication: Ristow lab plasmids (unpublished)

Backbone

• Vector backbone: pBluescript II
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human frataxin cDNA
• Alt name: hFX
• Alt name: FRX
• Alt name: frataxin
• Species: H. sapiens (human)
• Insert Size (bp): 428
• Mutation: contains cDNA coding sequence for amino acids 88-210 of the original human frataxin gene; changed isoleucine 154 to phenylalanine (mutation I154F localization corresponds to full-length protein)
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • AU1 (C terminal on insert)
  • HIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry