RG6-BAP1_E11
(Plasmid
#154025)
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PurposeBichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRG6
- Backbone size w/o insert (bp) 8137
- Total vector size (bp) 8310
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Vector typeMammalian Expression ; Splicing Reporter
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBAP1 Exon 11 Fused to DsRed1 and eGFP
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Alt nameBRCA1 associated protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)196
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GenBank IDNM_004656.4
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Entrez GeneBAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
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Tags
/ Fusion Proteins
- FLAG (N terminal on backbone)
- SV40 NLS (N terminal on backbone)
- DsRed1 (C terminal on backbone)
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer RG6_RT-PCR_Fwd (5'-CAAAGTGGAGGACCCAGTACC-3')
- 3′ sequencing primer RG6_RT-PCR_Rev (5'-GCGCATGAACTCCTTGATGAC-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pL164
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RG6-BAP1_E11 was a gift from Samuel Peña-Llopis (Addgene plasmid # 154025 ; http://n2t.net/addgene:154025 ; RRID:Addgene_154025) -
For your References section:
A BAP1 synonymous mutation results in exon skipping, loss of function and worse patient prognosis. Niersch J, Vega-Rubin-de-Celis S, Bazarna A, Mergener S, Jendrossek V, Siveke JT, Pena-Llopis S. iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173. eCollection 2021 Mar 19. 10.1016/j.isci.2021.102173 PubMed 33681728