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RG6-BAP1_E7
(Plasmid #154023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RG6
  • Backbone size w/o insert (bp) 8137
  • Total vector size (bp) 8265
  • Vector type
    Mammalian Expression ; Splicing Reporter
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAP1 Exon 7 Fused to DsRed1 and eGFP
  • Alt name
    BRCA1 associated protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    151
  • GenBank ID
    NM_004656.4
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • SV40 NLS (N terminal on backbone)
    • DsRed1 (C terminal on backbone)
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer RG6_RT-PCR_Fwd (5'-CAAAGTGGAGGACCCAGTACC-3')
  • 3′ sequencing primer RG6_RT-PCR_Rev (5'-GCGCATGAACTCCTTGATGAC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pL162

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RG6-BAP1_E7 was a gift from Samuel Peña-Llopis (Addgene plasmid # 154023 ; http://n2t.net/addgene:154023 ; RRID:Addgene_154023)

  • For your References section:

    A BAP1 synonymous mutation results in exon skipping, loss of function and worse patient prognosis. Niersch J, Vega-Rubin-de-Celis S, Bazarna A, Mergener S, Jendrossek V, Siveke JT, Pena-Llopis S. iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173. eCollection 2021 Mar 19. 10.1016/j.isci.2021.102173 PubMed 33681728
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