pBABE-hygro-BAP1-G312G-HA
(Plasmid #154022)

• Purpose: Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G
• Depositing Lab: Samuel Peña-Llopis
• Publication: Niersch et al iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173. eCollection 2021 Mar 19.

Backbone

• Vector backbone: pBABE-hygro
• Backbone size w/o insert (bp): 5219
• Total vector size (bp): 7424
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAP1
• Alt name: BRCA1 associated protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 2233
• Mutation: c.936T>G, p.G312G (Synonymous Mutation) by Site-Directed Mutagenesis
• GenBank ID: NM_004656.4
• Entrez Gene: BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
• Tag / Fusion Protein:
  • Hemagglutinin (HA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE-5' (5'-CTTTATCCAGCCCTCAC-3')
• 3′ sequencing primer: pBABE-3' (5'-ACCCTAACTGACACACATTCC-3')

Resource Information

• Supplemental Documents:
  • pBABE-hygro-BAP1-G312G-HA.gbk
• A portion of this plasmid was derived from a plasmid made by: Machida et al. J. Biol. Chem. 2009 (pcDNA3.1-BAP1) and mutated by Site-Directed Mutagenesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pL148 - This plasmid constitutively expresses HA-tagged BAP1 with a c.936T>G, p.G312G synonymous mutation, which is insensitive to knockdown by pMMP-puro-shBAP1.2132, targeting 5'-GAGUUCAUCUGCACCUUUA-3' BAP1 sequence.