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pBABE-hygro-BAP1-HA
(Plasmid #154020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone size w/o insert (bp) 5219
  • Total vector size (bp) 7424
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAP1
  • Alt name
    BRCA1 associated protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2233
  • GenBank ID
    NM_004656.4
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Tag / Fusion Protein
    • Hemagglutinin (HA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE-5' (5'-CTTTATCCAGCCCTCAC-3')
  • 3′ sequencing primer pBABE-3' (5'-ACCCTAACTGACACACATTCC-3')
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Machida et al. J. Biol. Chem. 2009 (pcDNA3.1-BAP1)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pL116 - This plasmid constitutively expresses HA-tagged full-length wild-type BAP1, which is insensitive to knockdown by pMMP-puro-shBAP1.2132, targeting 5'-GAGUUCAUCUGCACCUUUA-3' BAP1 sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-hygro-BAP1-HA was a gift from Samuel Peña-Llopis (Addgene plasmid # 154020 ; http://n2t.net/addgene:154020 ; RRID:Addgene_154020)

  • For your References section:

    A BAP1 synonymous mutation results in exon skipping, loss of function and worse patient prognosis. Niersch J, Vega-Rubin-de-Celis S, Bazarna A, Mergener S, Jendrossek V, Siveke JT, Pena-Llopis S. iScience. 2021 Feb 12;24(3):102173. doi: 10.1016/j.isci.2021.102173. eCollection 2021 Mar 19. 10.1016/j.isci.2021.102173 PubMed 33681728
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