frh-1-C
(Plasmid
#15401)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneL4440
- Backbone size w/o insert (bp) 2676
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFrataxin homologue
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Alt nameFrataxin
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SpeciesC. elegans (nematode)
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Insert Size (bp)103
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GenBank IDNC_003280
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Entrez Genefrh-1 (a.k.a. CELE_F59G1.7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
frh-1-C was a gift from Michael Ristow (Addgene plasmid # 15401 ; http://n2t.net/addgene:15401 ; RRID:Addgene_15401) -
For your References section:
Impaired respiration is positively correlated with decreased life span in Caenorhabditis elegans models of Friedreich Ataxia. Zarse K, Schulz TJ, Birringer M, Ristow M. FASEB J. 2007 Apr . 21(4):1271-5. 10.1096/fj.06-6994com PubMed 17215485