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Addgene

p3xFLAG SARS-CoV2 RdRp.co
(Plasmid #154008)

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Item Catalog # Description Quantity Price (USD)
Plasmid 154008 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    p3xFLAG-CMV-14
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6310
  • Total vector size (bp) 9159
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab
  • Alt name
    SARS-CoV2 RdRp
  • Species
    Synthetic
  • Insert Size (bp)
    2892
  • GenBank ID
    MN985325.1
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer 3xFLAG reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert DNA was synthesized by IDT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG SARS-CoV2 RdRp.co was a gift from Patrick Moore (Addgene plasmid # 154008 ; http://n2t.net/addgene:154008 ; RRID:Addgene_154008)

  • For your References section:

    Development and characterization of a new monoclonal antibody against SARS-CoV-2 NSP12 (RdRp). Meng W, Guo S, Cao S, Shuda M, Robinson-McCarthy LR, McCarthy KR, Shuda Y, Paniz Mondolfi AE, Bryce C, Grimes Z, Sordillo EM, Cordon-Cardo C, Li P, Zhang H, Perlman S, Guo H, Gao SJ, Chang Y, Moore PS. J Med Virol. 2023 Jan;95(1):e28246. doi: 10.1002/jmv.28246. Epub 2022 Nov 2. 10.1002/jmv.28246 PubMed 36271490
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