pCRL1
(Plasmid
#153997)
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Purpose(Empty Backbone) sgRNA in vitro transcription plasmid.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDR274
- Backbone size (bp) 2147
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Modifications to backboneAdded a construct to express sgRNA under a T7 promoter.
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Vector typeCRISPR ; sgRNA under a T7 promoter for in vitro transcription.
- Promoter T7
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRL1 was a gift from Shengdar Tsai (Addgene plasmid # 153997 ; http://n2t.net/addgene:153997 ; RRID:Addgene_153997) -
For your References section:
Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq. Lazzarotto CR, Nguyen NT, Tang X, Malagon-Lopez J, Guo JA, Aryee MJ, Joung JK, Tsai SQ. Nat Protoc. 2018 Nov;13(11):2615-2642. doi: 10.1038/s41596-018-0055-0. 10.1038/s41596-018-0055-0 PubMed 30341435
Map uploaded by the depositor.
