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SNAP-Dcp2
(Plasmid #153975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACGFP-c1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 5787
  • Modifications to backbone
    GFP replaced with SNAP tag using NheI and BspEI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRNA Decapping enzyme 2
  • Alt name
    Dcp2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1260
  • Entrez Gene
    DCP2 (a.k.a. NUDT20)
  • Tag / Fusion Protein
    • SNAP-C1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GGTGCAGGGCGACCTGGACG
  • 3′ sequencing primer GGTGTGGGAGGTTTTTTAAAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GFP-Dcp2 was a gift from Ross Buchan.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We amplified GFP-Dcp2 to obtain SNAP-Dcp2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNAP-Dcp2 was a gift from Gia Voeltz (Addgene plasmid # 153975 ; http://n2t.net/addgene:153975 ; RRID:Addgene_153975)

  • For your References section:

    Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles. Lee JE, Cathey PI, Wu H, Parker R, Voeltz GK. Science. 2020 Jan 31;367(6477). pii: 367/6477/eaay7108. doi: 10.1126/science.aay7108. 10.1126/science.aay7108 PubMed 32001628
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