pEGFP-IREShygro2
(Plasmid #153966)

• Purpose: Expresses EGFP in mammalian cells
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

Backbone

• Vector backbone: pIREShyg2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5788
• Total vector size (bp): 6565
• Modifications to backbone: N/A
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Alt name: N/A
• Species: Synthetic
• Insert Size (bp): 799
• Mutation: No mutation
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A NheI-BamHI fragment (containing EGFP) excised from pEGFP-C1 (Clontech) was inserted into the NheI-BamHI site of pIREShyg2 (Clontech)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry