prCOrev
(Plasmid #153961)

• Purpose: Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

Backbone

• Vector backbone: pBABEneo
• Backbone size w/o insert (bp): 5330
• Total vector size (bp): 7261
• Modifications to backbone: A promoter driving NeoR/KanR expression in pBABEneo was replaced with IVS-IRES
• Vector type: Mammalian Expression, Retroviral ; A reporter plasmid detecting DNA recombination
• Selectable markers: Neomycin (select with G418) ; NeoR/KanR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
• Alt name: N/A
• Species: Synthetic
• Insert Size (bp): 3151
• Mutation: No mutation
• Promoter: LTR
• Tag / Fusion Protein:
  • NLS-ZF (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified EGFP in this plasmid was originally derived from pDRGFP (Addgene #26475) provided by Maria Jasin

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry