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Addgene

pCAG-EGxxFP-PIGA
(Plasmid #153958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-EGxxFP (Addgene #50716)
  • Backbone manufacturer
    Masahito Ikawa
  • Backbone size w/o insert (bp) 6383
  • Total vector size (bp) 6784
  • Modifications to backbone
    N/A
  • Vector type
    Mammalian Expression ; A plasmid specific for EGxxFP assay
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIGA (a 401-bp fragment from intron 5 and exon 6)
  • Alt name
    N/A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    401
  • Mutation
    No mutation
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-EGxxFP-PIGA was a gift from Hiroyuki Konishi (Addgene plasmid # 153958 ; http://n2t.net/addgene:153958 ; RRID:Addgene_153958)

  • For your References section:

    Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53. Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H. Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064. 10.1016/j.celrep.2019.12.064 PubMed 31995758
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