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pDONR223 SARS-CoV-2 S 24nt-del_nostop
(Plasmid #153953)

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Item Catalog # Description Quantity Price (USD)
Plasmid 153953 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Rual et al, Genome Res. 14:2128-2135, 2004.
  • Backbone size w/o insert (bp) 5005
  • Vector type
    Gateway-compatible Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
    3795
  • Mutation
    Many synonymous changes due to codon optimization
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kim et al. 2020 can be accessed at https://doi.org/10.1016/j.cell.2020.04.011

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 SARS-CoV-2 S 24nt-del_nostop was a gift from Fritz Roth (Addgene plasmid # 153953 ; http://n2t.net/addgene:153953 ; RRID:Addgene_153953)

  • For your References section:

    A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951
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