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Addgene

pPB CAG Gfi1:FLAG-IRES-EGFP
(Plasmid #153945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac transposon
  • Backbone size w/o insert (bp) 7061
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gfi1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1305
  • Entrez Gene
    Gfi1 (a.k.a. Gfi-1, Pal-1, Pal1)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB CAG Gfi1:FLAG-IRES-EGFP was a gift from Luca Tiberi (Addgene plasmid # 153945 ; http://n2t.net/addgene:153945 ; RRID:Addgene_153945)

  • For your References section:

    Modeling medulloblastoma in vivo and with human cerebellar organoids. Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, Tiberi L. Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3. 10.1038/s41467-019-13989-3 PubMed 31996670
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