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Addgene

pEsg1-6K-Luc
(Plasmid #15394)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGV-BM2
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream region of mouse Esg1 gene
  • Alt name
    Esg1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6000
  • Entrez Gene
    Dppa5a (a.k.a. AA536857, Dppa5, Esg1, ecat2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGV-2U
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEsg1-6K-Luc was a gift from Shinya Yamanaka (Addgene plasmid # 15394 ; http://n2t.net/addgene:15394 ; RRID:Addgene_15394)

  • For your References section:

    Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5). Amano H, Itakura K, Maruyama M, Ichisaka T, Nakagawa M, Yamanaka S. BMC Dev Biol. 2006 . 6():11. 10.1186/1471-213X-6-11 PubMed 16504174
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