LB1361 (Bcl-2 promoter with MHS12)
(Plasmid
#15391)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepREP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10183
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Vector typeMammalian Expression, Bacterial Expression, Luciferase
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameB-cell lymphoma/leukemia-2
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Alt nameBcl-2
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SpeciesH. sapiens (human)
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Insert Size (bp)1563
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MutationBcl-2 full promoter with MHS12
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Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
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Tag
/ Fusion Protein
- Bcl-2-Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer pREP_fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LB1361 (Bcl-2 promoter with MHS12) was a gift from Linda Boxer (Addgene plasmid # 15391 ; http://n2t.net/addgene:15391 ; RRID:Addgene_15391) -
For your References section:
The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan H, Heckman CA, Boxer LM. Oncogene. 2007 Apr 19. 26(18):2635-41. 10.1038/sj.onc.1210061 PubMed 17043638
Map uploaded by the depositor.
