pGBW-m4092694
(Plasmid #153907)

• Purpose: Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein E. coli expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: pG9m-2 backbone
• Backbone size w/o insert (bp): 1968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 4928
• Mutation: wt
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: Promoter | t7 consensus from NEB, G is +1

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.

During Addgene quality control, an IS3 element and transposase were identified downstream from the SARS-CoV-2 fragment. Requesting scientists should validate that the element has not further transposed into essential elements prior to experimental use.