pGBW-m4092686
(Plasmid #153895)

• Purpose: Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein E. coli expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: pG9m-2 backbone
• Backbone size w/o insert (bp): 1968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 8319
• Mutation: Nearly native encoding;inactivating mutations in all ORFs and tIIs restriction sites removed with 5 base polyA tail
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: Promoter | t7 consensus from NEB, G is +1

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.