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Addgene

pQsupR-Mi2b
(Plasmid #15379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQsupR
  • Backbone manufacturer
    Smale Lab
  • Backbone size w/o insert (bp) 8290
  • Vector type
    Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    short hairpin RNA against mouse Mi-2 beta
  • Alt name
    Mi-2 beta
  • Alt name
    Mi-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    66
  • Entrez Gene
    Chd4 (a.k.a. 9530019N15Rik, D6Ertd380e, Mi-2beta, mKIAA4075)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BgIII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ccatggaattcgaacgctgacgtc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target sequence: GACTACGACCTGTTCAAGCAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQsupR-Mi2b was a gift from Stephen Smale (Addgene plasmid # 15379 ; http://n2t.net/addgene:15379 ; RRID:Addgene_15379)

  • For your References section:

    Selective and antagonistic functions of SWI/SNF and Mi-2beta nucleosome remodeling complexes during an inflammatory response. Ramirez-Carrozzi VR, Nazarian AA, Li CC, Gore SL, Sridharan R, Imbalzano AN, Smale ST. Genes Dev. 2006 Feb 1. 20(3):282-96. 10.1101/gad.1383206 PubMed 16452502
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