pGBW-m4252845
(Plasmid #153787)

• Purpose: Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein Mammalian expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: pTwist_CMV_Puro_6 with AarI site removed
• Backbone size w/o insert (bp): 6080
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 S (surface glycoprotein)
• Alt name: Spike protein
• Alt name: S protein
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 3815
• Mutation: wt
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tag / Fusion Protein:
  • Cleavable TEV;6xHis-003 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI reference sequence: 43740568