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His-FLAG-Med18 pBacPAK8
(Plasmid #15371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5538
  • Vector type
    Baculovirus construction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Med18
  • Alt name
    p28b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    687
  • Entrez Gene
    MED18 (a.k.a. SRB5, p28b)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1/Nhe1 (destroyed during cloning)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer Bac1
  • 3′ sequencing primer Bac2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-FLAG-Med18 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15371 ; http://n2t.net/addgene:15371 ; RRID:Addgene_15371)

  • For your References section:

    Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2003 Apr 25. 278(17):15123-7. 10.1074/jbc.C300054200 PubMed 12584197
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