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Addgene

F-MED28
(Plasmid #15368)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15368 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 N-FLAG/Hyg
  • Backbone manufacturer
    Invitrogen, modified by Conaway lab
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MED28
  • Alt name
    magicin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    537
  • Mutation
    N/A
  • GenBank ID
    NM_025205
  • Entrez Gene
    MED28 (a.k.a. 1500003D12Rik, EG1, magicin)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F-MED28 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15368 ; http://n2t.net/addgene:15368 ; RRID:Addgene_15368)

  • For your References section:

    A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC. Mol Cell. 2004 Jun 4. 14(5):685-91. 10.1016/j.molcel.2004.05.006 PubMed 15175163
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