F-Cdk8
(Plasmid #15366)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Conaway et al Trends Biochem Sci. 2005 May . 30(5):250-5.

Backbone

• Vector backbone: pcDNA3.1 N-FLAG/Hyg
• Backbone manufacturer: Invitrogen, modified at Conaway lab
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDK8
• Alt name: cyclin-dependent kinase 8
• Species: H. sapiens (human)
• Insert Size (bp): 1395
• Mutation: N/A
• GenBank ID: NM_001260
• Entrez Gene: CDK8 (a.k.a. IDDHBA, K35)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry