myc-Med4 pBacPAK8
(Plasmid
#15364)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5538
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Vector typeBaculovirus construction
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMed4
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Alt nameTRAP36
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SpeciesH. sapiens (human); N/A
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Entrez GeneMED4 (a.k.a. ARC36, DRIP36, HSPC126, TRAP36, VDRIP)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer Bac1
- 3′ sequencing primer Bac2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc-Med4 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15364 ; http://n2t.net/addgene:15364 ; RRID:Addgene_15364) -
For your References section:
A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2004 Feb 13. 279(7):5846-51. 10.1074/jbc.M312523200 PubMed 14638676