FLAG-Amida
(Plasmid
#15362)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5 FRT
- Backbone size w/o insert (bp) 5070
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCF3 (E2A) fusion partner
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Alt nameAmida
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SpeciesH. sapiens (human)
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Insert Size (bp)761
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Entrez GeneTFPT (a.k.a. FB1, INO80F, amida)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Amida was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15362 ; http://n2t.net/addgene:15362 ; RRID:Addgene_15362) -
For your References section:
A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. Jin J, Cai Y, Yao T, Gottschalk AJ, Florens L, Swanson SK, Gutierrez JL, Coleman MK, Workman JL, Mushegian A, Washburn MP, Conaway RC, Conaway JW. J Biol Chem. 2005 Dec 16. 280(50):41207-12. 10.1074/jbc.M509128200 PubMed 16230350