FLAG-TIP49b
(Plasmid #15358)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-12.

Backbone

• Vector backbone: pcDNA5 FRT
• Backbone size w/o insert (bp): 5070
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RuvB-like 2
• Alt name: TIP49b
• Species: H. sapiens (human)
• Insert Size (bp): 1391
• Entrez Gene: RUVBL2 (a.k.a. CGI-46, ECP-51, ECP51, INO80J, REPTIN, RVB2, TAP54-beta, TIH2, TIP48, TIP49B)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry