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pGBW-m4164342
(Plasmid #153553)

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Item Catalog # Description Quantity Price (USD)
Plasmid 153553 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pSF-CAG backbone NotI/NheI fragment [add Kozak, START, STOP]
  • Backbone size w/o insert (bp) 5192
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 S (surface glycoprotein)
  • Alt name
    Spike protein
  • Alt name
    S protein
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3758
  • Mutation
    Gordon et al recoded
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • accatg Kozak-START linker (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI reference sequence: YP_009724390.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4164342 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153553 ; http://n2t.net/addgene:153553 ; RRID:Addgene_153553)
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