Donor-PIGA-NeoR
(Plasmid #153549)

• Purpose: Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

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• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone

• Vector backbone: pAAV-2Aneo v2 (Addgene #80033)
• Backbone manufacturer: Konishi lab
• Backbone size w/o insert (bp): 4499
• Total vector size (bp): 6509
• Modifications to backbone: N/A
• Vector type: AAV, Cre/Lox ; Donor plasmid/Targeting vector
• Selectable markers: Neomycin (select with G418) ; NeoR/KanR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
• Alt name: N/A
• Species: H. sapiens (human)
• Insert Size (bp): 1991
• Mutation: a 3-bp mutation in exon 6
• Promoter: No promoter
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI for 5' arm cloning (not destroyed)
• 3′ cloning site: XhoI for 3' arm cloning (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry