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Donor-PIGA-NeoR
(Plasmid #153549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-2Aneo v2 (Addgene #80033)
  • Backbone manufacturer
    Konishi lab
  • Backbone size w/o insert (bp) 4499
  • Total vector size (bp) 6509
  • Modifications to backbone
    N/A
  • Vector type
    AAV, Cre/Lox ; Donor plasmid/Targeting vector
  • Selectable markers
    Neomycin (select with G418) ; NeoR/KanR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
  • Alt name
    N/A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1991
  • Mutation
    a 3-bp mutation in exon 6
  • Promoter No promoter
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI for 5' arm cloning (not destroyed)
  • 3′ cloning site XhoI for 3' arm cloning (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Donor-PIGA-NeoR was a gift from Hiroyuki Konishi (Addgene plasmid # 153549 ; http://n2t.net/addgene:153549 ; RRID:Addgene_153549)

  • For your References section:

    Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53. Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H. Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064. 10.1016/j.celrep.2019.12.064 PubMed 31995758
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