Donor-PIGAmut
(Plasmid #153548)

• Purpose: Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

Backbone

• Vector backbone: pBSII-KS(+)
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4895
• Modifications to backbone: N/A
• Vector type: Donor plasmid
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
• Alt name: N/A
• Species: H. sapiens (human)
• Insert Size (bp): 1991
• Mutation: a 3-bp mutation in exon 6
• Promoter: No promoter
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7, M13 fwd
• 3′ sequencing primer: T3, M13 rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry