LIC-Z-Y-L
(Plasmid
#153546)
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Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman TCR ζ-Chain
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SpeciesH. sapiens (human)
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Mutationwith 6 Y in 3 ITAMs mutated to L
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Entrez GeneCD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LIC-Z-Y-L was a gift from Katharina Gaus (Addgene plasmid # 153546 ; http://n2t.net/addgene:153546 ; RRID:Addgene_153546) -
For your References section:
Clustering of the zeta-Chain Can Initiate T Cell Receptor Signaling. Ma Y, Lim YJ, Benda A, Lou J, Goyette J, Gaus K. Int J Mol Sci. 2020 May 15;21(10). pii: ijms21103498. doi: 10.3390/ijms21103498. 10.3390/ijms21103498 PubMed 32429097