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Addgene

LIC-Z-delCry2
(Plasmid #153545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153545 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human TCR ζ-Chain
  • Species
    H. sapiens (human)
  • Mutation
    with Cry2 sequence deleted from LIC-Z
  • Entrez Gene
    CD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIC-Z-delCry2 was a gift from Katharina Gaus (Addgene plasmid # 153545 ; http://n2t.net/addgene:153545 ; RRID:Addgene_153545)

  • For your References section:

    Clustering of the zeta-Chain Can Initiate T Cell Receptor Signaling. Ma Y, Lim YJ, Benda A, Lou J, Goyette J, Gaus K. Int J Mol Sci. 2020 May 15;21(10). pii: ijms21103498. doi: 10.3390/ijms21103498. 10.3390/ijms21103498 PubMed 32429097
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