Donor-PIGA
(Plasmid
#153530)
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PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSII-KS(+)
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 4895
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Modifications to backboneN/A
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Vector typeDonor plasmid
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePIGA (a 1,991-bp fragment from intron 5 and exon 6)
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Alt nameN/A
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SpeciesH. sapiens (human)
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Insert Size (bp)1991
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MutationNo mutation
- Promoter No promoter
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Tag
/ Fusion Protein
- N/A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7, M13 fwd
- 3′ sequencing primer T3, M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Donor-PIGA was a gift from Hiroyuki Konishi (Addgene plasmid # 153530 ; http://n2t.net/addgene:153530 ; RRID:Addgene_153530) -
For your References section:
Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53. Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H. Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064. 10.1016/j.celrep.2019.12.064 PubMed 31995758