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Addgene

pHes7-Achilles-Hes7
(Plasmid #153528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2974
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hes7
  • Alt name
    hes family bHLH transcription factor 7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8952
  • Entrez Gene
    Hes7 (a.k.a. bHLHb37)
  • Promoter mouse Hes7 promoter
  • Tag / Fusion Protein
    • Achilles (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHes7-Achilles-Hes7 was a gift from Ryoichiro Kageyama (Addgene plasmid # 153528 ; http://n2t.net/addgene:153528 ; RRID:Addgene_153528)

  • For your References section:

    Coupling delay controls synchronized oscillation in the segmentation clock. Yoshioka-Kobayashi K, Matsumiya M, Niino Y, Isomura A, Kori H, Miyawaki A, Kageyama R. Nature. 2020 Apr;580(7801):119-123. doi: 10.1038/s41586-019-1882-z. Epub 2020 Jan 8. 10.1038/s41586-019-1882-z PubMed 31915376
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