tFucci(SA)5
(Plasmid #153520)

• Purpose: New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.
• Depositing Lab: Atsushi Miyawaki
• Publication: Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.1247/csf.23028.

Backbone

• Vector backbone: pPBbsr2
• Backbone manufacturer: Sanger Institute
• Total vector size (bp): 8905
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tFucci(SA)5.
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2151
• GenBank ID: LC334437
• Promoter: CAG
• Tag / Fusion Protein:
  • AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: GATCCGCCACCATGGAGAACGTCAGGAG

Resource Information

• Supplemental Documents:
  • tFucci(SA)5.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2020.03.30.015156 for bioRxiv preprint.