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Addgene

tFucci(SA)5
(Plasmid #153520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBbsr2
  • Backbone manufacturer
    Sanger Institute
  • Total vector size (bp) 8905
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tFucci(SA)5.
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2151
  • GenBank ID
    LC334437
  • Promoter CAG
  • Tag / Fusion Protein
    • AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer GATCCGCCACCATGGAGAACGTCAGGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.03.30.015156 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tFucci(SA)5 was a gift from Atsushi Miyawaki (Addgene plasmid # 153520 ; http://n2t.net/addgene:153520 ; RRID:Addgene_153520)

  • For your References section:

    Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A. Cell Struct Funct. 2023 Jun 30. doi: 10.1247/csf.23028. 10.1247/csf.23028 PubMed 37394513
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