pAGM9121_TwinStrep
(Plasmid
#153513)
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PurposeTwinStrep C-terminal Tag Donor Plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153513 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM9121
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTwinStrep
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM9121_TwinStrep was a gift from Sylvestre Marillonnet (Addgene plasmid # 153513 ; http://n2t.net/addgene:153513 ; RRID:Addgene_153513) -
For your References section:
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases. Pullmann P, Knorrscheidt A, Munch J, Palme PR, Hoehenwarter W, Marillonnet S, Alcalde M, Westermann B, Weissenborn MJ. Commun Biol. 2021 May 12;4(1):562. doi: 10.1038/s42003-021-02076-3. 10.1038/s42003-021-02076-3 PubMed 33980981