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Addgene

pJJBAD82
(Plasmid #15348)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Invitrogen but modified
  • Backbone size w/o insert (bp) 4771
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DnaE C-intein(CEN)
  • Alt name
    Intein
  • Species
    Synechocystis sp. PCC6803
  • Insert Size (bp)
    117
  • Mutation
    F to E mutation
  • Tag / Fusion Protein
    • GB1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBAD-fw
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJBAD82 was a gift from Hideo Iwai (Addgene plasmid # 15348 ; http://n2t.net/addgene:15348 ; RRID:Addgene_15348)

  • For your References section:

    Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H, Zuger S, Jin J, Tam PH. FEBS Lett. 2006 Mar 20. 580(7):1853-8. 10.1016/j.febslet.2006.02.045 PubMed 16516207
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